GlycoSpectrumScan

*    GlycoSpectrumScan scans a mass spectrum for all possible glycopeptides.
*    The tool uses peptide mass/composition and glycan masses/compositions as inputs.
*    Generates all possible glycoform combinations.      Abstract
Select glycosylation type
 Click here for a previously processed query
N-linked O-linked
STEP 1. GLYCAN DATASETS
STEP 2. PEPTIDE / PROTEIN DATASETS

1) Click here to upload a glycan compositions file


2) If you want to save the uploaded glycan compositions file for future use,Click on

Click here to
Upload one or more         Peptide Masses [M]

OR
Click here to
Upload one or more          Peptide Sequences

STEP 3. MASS SPECTROMETER DATASETS

Use peptideMass to generate all possible peptides of a protein of interest

Enter a Protein Sequence or a Swiss-Prot/TrEMBL ID or AC:
      

Enzyme :

 *  Allow for missed cleavages.
 *  Display the peptides with a mass

  •     Bigger than and smaller than Dalton
  •     Sorted by peptide masses OR in chronological order in the protein

 *    The peptide masses are with cysteines treated with :       

    with acrylamide adducts with methionines oxidized

Upload MS data file (m/z vs intensity values)

File format?
Select charge state(s) (z)



To select multiple charge states , press "Ctrl" button and click on the charge state

OR
 Process for all charge states
Ion mode and adducts
positive negative neutral
[M+H]x+ [M-H]x- [M]

 

Mass tolerance :+/- Dalton

Peptide masses are : average / monoisotopic
STEP 4.